pCtrlki-kanMX6 vector (V008124)

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LOCUS       40924_13765        4861 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCtrlki-kanMX6 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Takeda J, Taoka M, Isobe T.
  TITLE     Cloning vector pCtrlki-kanMX6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Takeda J, Taoka M, Isobe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2011) Contact:Jun Takeda Tokyo Metropolitan 
            University, Graduate School of Science and Engineering; 1-1 
            Minami-osawa, Hachioji, Tokyo 192-0397, Japan
REFERENCE   3  (bases 1 to 4861)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-APR-2011) Contact:Jun Takeda Tokyo Metropolitan University, 
            Graduate School of Science and Engineering; 1-1 Minami-osawa, 
            Hachioji, Tokyo 192-0397, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    231..266
                     /label=Multiple cloning site 1
                     /note="Multiple cloning site 1"
     polyA_signal    391..510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            556..1912
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     misc_feature    1947..1976
                     /label=Multiple cloning site 2
                     /note="Multiple cloning site 2"
     primer_bind     complement(1990..2006)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2219..2674
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2956..3060
                     /label=AmpR promoter
     CDS             3061..3918
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4092..4680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"