pCTAGII-GUSPlus vector (V008126)

LOCUS       40924_13735       13372 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCTAGII-GUSPlus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13372)
  AUTHORS   Thomson JG.
  TITLE     pCTAGII binary vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13372)
  AUTHORS   Thomson JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2018) Crop Improvement and Genetics Reseach Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 13372)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13372)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JAN-2018) Crop Improvement and Genetics Reseach Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    35..352
                     /label=Zea mays Ds transposon
                     /note="Zea mays Ds transposon"
     protein_bind    complement(360..393)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    435..540
                     /label=MRS recognition site for ParA recombinase
                     /note="MRS recognition site for ParA recombinase"
     misc_feature    574..692
                     /label=RS2 recognition site for cinH recombinase
                     /note="RS2 recognition site for cinH recombinase"
     terminator      complement(760..1012)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1076..1864)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(1889..3169)
                     /label=codA
                     /note="E. coli cytosine deaminase"
     regulatory      complement(3177..3978)
                     /label=Arabidopsis ubiquitin 10 promoter and 5' intron
                     /note="Arabidopsis ubiquitin 10 promoter and 5' intron"
                     /regulatory_class="promoter"
     intron          complement(3193..3480)
                     /note="AtUbi10 5' intron"
     intron          4109..4298
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             4293..6116
                     /label=GUSPlus(TM)
                     /note="beta-glucuronidase"
     CDS             6123..6140
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      6172..6424
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6570..6608
                     /label=PhiC31 attP recognition site
                     /note="PhiC31 attP recognition site"
     misc_feature    6628..6881
                     /label=Zea mays Ds transposon
                     /note="Zea mays Ds transposon"
     misc_feature    complement(6969..6993)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7418..8209
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      8299..8887
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9073..9213)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9557..9751)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9820..10884)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11321..11947)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(13247..13271)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(13327..13344)
                     /label=6xHis
                     /note="6xHis affinity tag"