pCTAG7 vector (V008127)

LOCUS       40924_13730       11050 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCTAG7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Dasgupta K, Thilmony R.
  TITLE     The Overexpression of Myb Transcription Factors from Plum and Citrus
            Produce Differential Patterns of Anthocyanin Accumulation in 
            Transgenic Tobacco
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   Dasgupta K, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2015) Crop Improvement and Utilization, USDA, 800 
            Buchanan Street, Albany, CA 94710, USA
REFERENCE   3  (bases 1 to 11050)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11050)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2015) Crop Improvement and Utilization, USDA, 800 Buchanan 
            Street, Albany, CA 94710, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1300..1926
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2363..3427
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3496..3690
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4034..4174
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4360..4948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5038..5829)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6254..6278
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_recomb     6576..6632
                     /label=phiC31 attP57 recognition site
                     /note="phiC31 attP57 recognition site"
     misc_recomb     6695..6742
                     /label=Bxb1 attP52 recognition site
                     /note="Bxb1 attP52 recognition site"
     misc_recomb     6799..6917
                     /label=CinH resolution site
                     /note="CinH resolution site"
     regulatory      6933..7747
                     /note="Arabidopsis ubiquitin 10 promoter; AtUbi10"
                     /regulatory_class="promoter"
     CDS             7749..9029
                     /label=codA
                     /note="E. coli cytosine deaminase"
     CDS             9054..9842
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      9906..10158
                     /label=nopaline synthase (nos) polyA terminator
                     /note="nopaline synthase (nos) polyA terminator"
                     /regulatory_class="terminator"
     terminator      9906..10158
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_recomb     10215..10333
                     /label=CinH resolution site
                     /note="CinH resolution site"
     misc_recomb     10383..10488
                     /label=ParA resolution site
                     /note="ParA resolution site"
     protein_bind    10530..10563
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    10898..10922
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10953..10970
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    11026..11050
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"