pCTAG6 vector (V008128)

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LOCUS       40924_13725       11439 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCTAG6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Thomson JG.
  TITLE     pCTAG6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11439)
  AUTHORS   Thomson JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2018) Crop Improvement and Genetics Reseach Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 11439)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JAN-2018) Crop Improvement and Genetics Reseach Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    35..351
                     /label=Zea mays Ds transposon
                     /note="Zea mays Ds transposon"
     protein_bind    complement(360..393)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    435..540
                     /label=MRS recognition site for ParA recombinase
                     /note="MRS recognition site for ParA recombinase"
     misc_feature    574..692
                     /label=RS2 recognition site for CinH recombinase
                     /note="RS2 recognition site for CinH recombinase"
     regulatory      complement(726..1108)
                     /label=Arabidopsis ubiquitin 10 transcription terminator
                     /note="Arabidopsis ubiquitin 10 transcription terminator"
                     /regulatory_class="terminator"
     CDS             complement(1124..1912)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(1937..3217)
                     /label=codA
                     /note="E. coli cytosine deaminase"
     regulatory      complement(3229..4039)
                     /label=Arabidopsis Ubiquitin 10 promoter and 5' intron
                     /note="Arabidopsis Ubiquitin 10 promoter and 5' intron"
                     /regulatory_class="promoter"
     intron          complement(3245..3532)
                     /note="Arabidopsis 5' enhancing intron"
     misc_recomb     4055..4106
                     /note="attB recognition site for Bxb1 site-specific
                     recombinase"
     misc_recomb     4166..4222
                     /note="attP recognition site for phiC31 site-specific 
                     recombinase"
     misc_feature    4232..4501
                     /label=Zea mays Ds transposon
                     /note="Zea mays Ds transposon"
     misc_feature    complement(5035..5059)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5484..6275
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6365..6953
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7139..7279)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7623..7817)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7886..8950)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9387..10013)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(11313..11337)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(11393..11410)
                     /label=6xHis
                     /note="6xHis affinity tag"