pCS2-olCre vector (V008146)

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LOCUS       40924_13610        5214 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cre expression vector pCS2-olCre DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Kishimoto K.
  TITLE     Two useful site-specific recombination system VCre/VloxP and 
            SCre/SloxP in Medaka
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Kishimoto K, Kinoshita M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2015) Contact:Kenta Kishimoto Kyoto University, 
            Graduate School of Agriculture; Kitashirakawa Oiwaketyo, Sakyo, 
            Kyoto 606-8502, Japan URL :http://www.kais.kyoto-u.ac.jp/english/
REFERENCE   3  (bases 1 to 5214)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2015) Contact:Kenta Kishimoto Kyoto University, Graduate 
            School of Agriculture"; volume: " Kitashirakawa Oiwaketyo, Sakyo, 
            Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.kais.kyoto-u.ac.jp/english"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    127..153
                     /gene="oCre"
                     /note="NLS; PPPKRPRLD"
     CDS             190..1218
                     /label=Cre
                     /note="site-specific recombinase"
     promoter        complement(1241..1258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(1263..1397)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1510..1528)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1549..1565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1573..1589)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1597..1627)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1642..1663)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1951..2539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2713..3570)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3571..3675)
                     /label=AmpR promoter
     rep_origin      complement(3701..4156)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4230..5214
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"