pCS167 vector (V008154)

LOCUS       V008154                12189 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008154
VERSION     V008154
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Wang G, Jones H, Doherty A, Yu X, Ma Y, Sun Y, Du W, Wang C, Xia L.
  TITLE     Generation of marker- and backbone-free transgenic wheat plants via
            Agrobacterium-mediated transformation based on the pCLEAN dual
            binary vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12189)
  AUTHORS   Wang G, Jones H, Doherty A, Yu X, Ma Y, Sun Y, Du W, Wang C, Xia L.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2015) Institute of Crop Sciences, Chinese Academy
            of Agricultural Sciences, 12 Zhongguancun Street, Beijing, Beijing
            100081, China
REFERENCE   3  (bases 1 to 12189)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUN-2015) Institute of Crop Sciences, Chinese Academy of
            Agricultural Sciences, 12 Zhongguancun Street, Beijing, Beijing
            100081, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..25)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(43..59)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(67..83)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(91..121)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(136..157)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        404..432
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             480..1667
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             2169..3314
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             4698..5666
                     /label="RepA"
                     /note="replication protein of plasmid pSa"
     CDS             6064..6711
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     oriT            7395..7504
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             7537..7905
                     /label="traJ"
                     /note="oriT-recognizing protein"
     rep_origin      8035..8746
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    9246..9270
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    9291..9415
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(9459..9761)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(10106..10762)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(10816..10846)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(10871..10995)
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    11032..11056
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    11057..11080
                     /note="right border overdrive sequence (Thole et al.,
                     (2007) Plant Physiol 145:1211-1219)"
     CDS             11294..12094
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"