pCS vector (V008155)

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LOCUS       V008155                 8420 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008155
VERSION     V008155
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
   PUBMED   21366919
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2011"; volume: "4"; issue: "1"; pages: "44"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Crop Improvement and Utilization Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(149..308)
                     /label="CaMV35S polyA terminator"
                     /note="CaMV35S polyA terminator"
                     /regulatory_class="terminator"
     CDS             complement(366..1202)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     transit_peptide complement(1203..1304)
                     /gene="Sul1"
                     /note="pea rbcS ribulose-bisphosphate carboxylase small
                     subunit transit peptide for plastid targeting"
     promoter        complement(1311..1982)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    2142..2166
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3467..4093
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4530..5594
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      5663..5857
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6201..6341
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6527..7115)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7205..7996)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"