pBIG-5b vector (V009183)

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LOCUS       V009183                 6831 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009183
VERSION     V009183
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Kulman JD.
  TITLE     Mammalian expression vector for doxycycline-regulated production of
            enzymatically biotinylated proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Kulman JD.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2014) Puget Sound Blood Center Research Institute,
            Puget Sound Blood Center, 1551 Eastlake Ave E #100, Seattle, WA
            98102, USA
REFERENCE   3  (bases 1 to 6831)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JUN-2014) Puget Sound Blood Center Research Institute, Puget
            Sound Blood Center, 1551 Eastlake Ave E #100, Seattle, WA 98102,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(29..991)
                     /label="BirA"
                     /note="E. coli biotin protein ligase"
     CDS             complement(1004..1027)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     regulatory      1027..1036
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1175..1445
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        1478..1515
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    1654..1686
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1693..1734
                     /codon_start=1
                     /product="biotin acceptor peptide"
                     /label="biotin acceptor peptide"
                     /note="contains lysine residue to which d-biotin is
                     enzymatically conjugated by an isopeptide linkage"
                     /protein_id="AIR07421.1"
                     /translation="MNDIFEAQKIEWH"
     misc_feature    1764..2350
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2405..3121
                     /label="EGFP"
                     /note="enhanced GFP"
     intron          3155..3727
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             3731..3853
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    3924..3979
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(4541..5129)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5303..6160)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6161..6265)
                     /label="AmpR promoter"
     polyA_signal    6696..6830
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"