pBIB-HYG vector (V009190)

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LOCUS       40924_6407       11619 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pBIB-HYG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Condori JM, Medina-Bolivar F.
  TITLE     Vector for transient expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   Condori JM, Medina-Bolivar F.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2012) Molecular Cloning, GeneCoMe Biotech LC, P.O.
            Box 19538, Jonesboro, AR 72403, USA
REFERENCE   3  (bases 1 to 11619)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11619)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-SEP-2012) Molecular Cloning, GeneCoMe Biotech LC, P.O. Box 
            19538, Jonesboro, AR 72403, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(926..1634)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(2639..2839)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     CDS             complement(2877..3899)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(3953..4132)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      4588..4840
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4849..4865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5483..5507)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(7106..8251)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(8553..9344)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(9535..10304)
                     /note="similar to transposable element IS1 in INSD
                     accession X58999; IS1"
     mobile_element  9536..10303
                     /mobile_element_type="insertion sequence:IS1"
                     /label=insertion sequence:IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(11004..11614)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"