pBHRF-108 vector (V009196)

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LOCUS       40924_6347        7024 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Transformation vector pBHRF-108, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Moody LA, Kelly S, Coudert Y, Nimchuk ZL, Harrison CJ, Langdale JA.
  TITLE     Somatic hybridization provides segregating populations for the 
            identification of causative mutations in sterile mutants of the moss
            Physcomitrella patens
  JOURNAL   New Phytol. (2018) In press
  PUBMED    29498048
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Schaefer DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Institute of Biology, University of 
            Neuchatal, Rue Emile-Argand 11, Neuchatal CH-2000, Switzerland
REFERENCE   3  (bases 1 to 7024)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7024)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2018) Institute of Biology, University of Neuchatal, Rue 
            Emile-Argand 11, Neuchatal CH-2000, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    182..1293
                     /note="108 targeting locus for ectopic integration of 
                     transgenes"
     protein_bind    1363..1396
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1599..1944
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1977..3002
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     regulatory      3018..3198
                     /label=35S terminator
                     /note="35S terminator"
                     /regulatory_class="terminator"
     protein_bind    complement(3276..3309)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3366..4364
                     /note="108 targeting locus for ectopic integration of 
                     transgenes"
     promoter        complement(4383..4401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4416..4432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4440..4456)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4464..4494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4509..4530)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4818..5406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5580..6437)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6438..6542)
                     /label=AmpR promoter
     rep_origin      6569..7024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"