pBGT1 vector (V009204)

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LOCUS       40924_6337       11160 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBGT1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Lukacsovich T, Hamada N, Miyazaki S, Kimpara A, Yamamoto D.
  TITLE     A new versatile gene-trap vector for insect transgenics
  JOURNAL   Arch. Insect Biochem. Physiol. 69 (4), 168-175 (2008)
  PUBMED    18949801
REFERENCE   2  (bases 1 to 11160)
  AUTHORS   Lukacsovich T, Hamada N, Yamamoto D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2008) Developmental and Cell Biology, University 
            of California Irvine, Rm 4444, McGaugh Hall, Irvine, CA 92697-2300, 
            USA
REFERENCE   3  (bases 1 to 11160)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11160)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch. 
            Insect Biochem. Physiol."; date: "2008"; volume: "69"; issue: "4"; 
            pages: "168-175"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2008) Developmental and Cell Biology, University of 
            California Irvine, Rm 4444, McGaugh Hall, Irvine, CA 92697-2300, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_structure  1098..2149
                     /label=PiggyBac right end
                     /note="PiggyBac right end"
     misc_feature    2169..2187
                     /label=splice acceptor site
                     /note="splice acceptor site"
     CDS             2207..4849
                     /label=GAL4
                     /note="GAL4 transcriptional activator"
     regulatory      5106..5348
                     /label=hsp70T
                     /note="hsp70T"
                     /regulatory_class="terminator"
     polyA_signal    complement(5385..5506)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5630..6307)
                     /label=Timer
                     /note="green-to-red fluorescent timer derivative of DsRed 
                     (Terskikh et al., 2000)"
     regulatory      complement(6360..6565)
                     /label=3xP3-hsp70
                     /note="3xP3-hsp70"
                     /regulatory_class="promoter"
     regulatory      6618..7335
                     /label=ie+hr
                     /note="ie+hr"
                     /regulatory_class="promoter"
     CDS             7350..8066
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    8069..8077
                     /label=splice donor site
                     /note="splice donor site"
     misc_structure  8079..8757
                     /label=PiggyBac left end
                     /note="PiggyBac left end"
     primer_bind     complement(8939..8955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8963..8979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8987..9017)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9032..9053)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9341..9929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10103..10960)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10961..11065)
                     /label=AmpR promoter