pBGSC6 vector (V009205)

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LOCUS       V009205                 3856 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009205
VERSION     V009205
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Zeigler DR.
  TITLE     Sequence of pBGSC6, a simple integration vector for Gram-positive
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Zeigler DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2006) Bacillus Genetic Stock Center, The Ohio
            State University, 484 W. 12th Ave., Columbus, OH 43210, USA
REFERENCE   3  (bases 1 to 3856)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2006) Bacillus Genetic Stock Center, The Ohio State
            University, 484 W. 12th Ave., Columbus, OH 43210, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(233..289)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(290..306)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             951..1598
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             2055..2912
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3086..3674
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"