pBGEN02-ALSdm vector (V009209)

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LOCUS       V009209                10549 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009209
VERSION     V009209
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Cloning vectors for achieving high levels of transgene expression in
            plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Kanoria S, Burma PK.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2011) Genetics, University of Delhi, South Campus,
            Benito Juarez Road, New Delhi, New Delhi 110021, India
REFERENCE   3  (bases 1 to 10549)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2011) Genetics, University of Delhi, South Campus, Benito
            Juarez Road, New Delhi, New Delhi 110021, India"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1813
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2250..3314
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3383..3577
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3921..4063
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4249..4837)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    5814..5838
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6152..6185
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6595..6641
                     /label="minimal CaMV 35S promoter"
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             6657..8666
                     /gene="ALS"
                     /label="Acetolactate synthase, chloroplastic"
                     /note="Acetolactate synthase, chloroplastic from
                     Arabidopsis thaliana. Accession#: P17597"
     regulatory      8686..8902
                     /label="35S"
                     /note="35S"
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    8919..8952
                     /label="loxP"
                     /note="loxP"
     protein_bind    complement(8919..8952)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     misc_feature    9206..9471
                     /label="multiple cloning site 2 (outside loxP)"
                     /note="multiple cloning site 2 (outside loxP)"
     promoter        9544..9890
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    9921..9941
                     /note="multiple cloning site 1 (between 35S promoter and
                     polyA)"
     misc_feature    10143..10240
                     /note="multiple cloning site 3 (for cloning another
                     transgene)"
     misc_feature    10413..10437
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"