pBgal vector (V009210)

Download GeneBank File(.gb)

LOCUS       40924_6307        5651 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBgal, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Lin SL.
  TITLE     Promoterless lacZ reporter gene in Salmonella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Lin SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-1999) Molecular and Cellular Biology, VION 
            Pharmaceuticals, Inc., 4 Science Park, New Haven, CT 06511, USA
REFERENCE   3  (bases 1 to 5651)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5651)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-OCT-1999) Molecular and Cellular Biology, VION Pharmaceuticals, 
            Inc., 4 Science Park, New Haven, CT 06511, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..63
                     /label=AspA
                     /note="AspA"
                     /regulatory_class="terminator"
     misc_feature    68..104
                     /label=polylinker
                     /note="polylinker"
     regulatory      138..141
                     /regulatory_class="ribosome_binding_site"
     CDS             163..180
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             184..216
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             220..243
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             274..3318
                     /label=lacZ
                     /note="beta-galactosidase"
     terminator      3531..3617
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3709..3736
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3756..3847
                     /label=AmpR promoter
     CDS             3848..4705
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4879..5467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"