Basic Vector Information
- Vector Name:
- pBG13
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3986 bp
- Type:
- Cloning vector
- Replication origin:
- R6K γ ori
- Source/Author:
- Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM.
- Promoter:
- EM7
pBG13 vector Map
pBG13 vector Sequence
LOCUS 40924_6262 3986 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pBG13, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3986) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE A Tn7-based device for calibrated heterologous gene expression in Pseudomonas putida JOURNAL ACS Synth Biol (2015) In press PUBMED 26133359 REFERENCE 2 (bases 1 to 3986) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE Direct Submission JOURNAL Submitted (15-JUN-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3986) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE Direct Submission JOURNAL Submitted (31-AUG-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain REFERENCE 4 (bases 1 to 3986) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE Direct Submission JOURNAL Submitted (13-NOV-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain REFERENCE 5 (bases 1 to 3986) TITLE Direct Submission REFERENCE 6 (bases 1 to 3986) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth Biol (2015) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (15-JUN-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (31-AUG-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain" COMMENT SGRef: number: 4; type: "Journal Article"; journalName: "Submitted (13-NOV-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain" COMMENT SGRef: number: 5; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## On Nov 13, 2015 this sequence version replaced KT192137.2. FEATURES Location/Qualifiers source 1..3986 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 11..58 /label=EM7 promoter /note="synthetic bacterial promoter" misc_feature 83..159 /label=BCD2 bicistronic linker /note="BCD2 bicistronic linker" RBS 151..159 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 178..891 /codon_start=1 /label=superfolder GFP /note="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /translation="IHKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" misc_feature 895..951 /label=MCS /note="pUC18/19 multiple cloning site" terminator 994..1088 /label=lambda t0 terminator /note="transcription terminator from phage lambda" misc_feature 1093..1291 /label=Tn7R transposase recognition site /note="Tn7R transposase recognition site" CDS 1401..2213 /codon_start=1 /label=KanR /note="aminoglycoside phosphotransferase" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" oriT 2372..2480 /label=oriT /note="incP origin of transfer" rep_origin 2499..2887 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" mobile_element 2899..3064 /label=Tn7L /note="mini-Tn7 element (left end of the Tn7 transposon)" terminator complement(3078..3164) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(3233..3763) /codon_start=1 /label=GmR /note="gentamycin acetyltransferase" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3952..3980) /label=Pc promoter /note="class 1 integron promoter"
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