pBE vector (V009237)

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LOCUS       40924_6167        5497 bp DNA     circular SYN 17-DEC-2018
DEFINITION  EGFP expression vector pBE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Colas J-F., Schoenwolf GC.
  TITLE     Assessing the contribution of gene products to the form-shaping 
            events of neurulation: a transgenic approach in chick
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Colas J-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2003) Neurobiology and Anatomy, University of 
            Utah, 20 North 1900 East MREB401, Salt Lake City, UT 84132-3401, USA
REFERENCE   3  (bases 1 to 5497)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5497)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-FEB-2003) Neurobiology and Anatomy, University of Utah, 20 North
            1900 East MREB401, Salt Lake City, UT 84132-3401, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..334
                     /label=chicken beta-actin promoter
     intron          335..1344
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     exon            1345..1398
                     /note="Oryctolagus cuniculus beta1-globin gene, GenBank 
                     Accession Number K03256; exon 3"
     CDS             1443..2159
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2285..2406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2413..2868)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2895..2999
                     /label=AmpR promoter
     promoter        3001..3358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3393..4184
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4419..4466
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4795..5383
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"