pBCGL1 vector (V009246)

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LOCUS       V009246                 8202 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009246
VERSION     V009246
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Govan VA, Christensen ND, Berkower C, Jacobs WR Jr., Williamson A-L.
  TITLE     Recombinant BCG expressing cottontail rabbit papillomavirus (CRPV)
            L1 gene provides partial protection in rabbits challenged against
            CRPV
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Govan VA, Christensen ND, Berkower C, Jacobs WR Jr., Williamson A-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2005) Division of Medical Virology, Institute of
            Infectious Diseases and Molecular Medicine, Anzio Road, Observatory,
            Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 8202)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8202)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2005) Division of Medical Virology, Institute of Infectious
            Diseases and Molecular Medicine, Anzio Road, Observatory, Cape Town
            7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1877..2689
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             5698..6330
                     /gene="tmk"
                     /label="Thymidylate kinase"
                     /note="Thymidylate kinase from Mycobacterium tuberculosis
                     (strain ATCC 25618 / H37Rv). Accession#: P9WKE1"
     CDS             6556..8073
                     /codon_start=1
                     /gene="L1"
                     /product="CRPV L1 major capsid protein"
                     /label="L1"
                     /note="derived from cottontail rabbit papillomavirus"
                     /protein_id="AAX37308.1"
                     /translation="MAVWLSTQNKFYLPPQPVTKIPSTDEYVTRTNVFYYASSDRLLTV
                     GHPYYEIRDKGTMLVPKVSPNQYRVFRIKLPDPNKFAFGDKQLYDPEKERLVWCLRGIE
                     VNRGQPLGVSVTGNPIFNKFDDVENPTKYYNNHADQQDYRKSMAFDPKQVQLLMLGCVP
                     ATGEHWAQAKQCAEDPPQQTDCPPIELVNTVIEDGDMCEIGFGAMDHKTLQASLSEVPL
                     ELAQSISKYPDYLKMQKDQFGDSMFFYARREQMYARHFFSRAGGDKENVKSRAYIKRTQ
                     MQGEANANIATDNYCITPSGSLVSSDSQVFNRAYWLQKAQGMNNGVCWDNQIFVTVVDN
                     TRGTILSLVTKSKEQIKKTHGKTVHFSSYLRHVEEYELQFVLQLCKVKLTPENLSYLHS
                     MHPTIIDNWQLSVSAQPSGTLEDQYRYLQSIATKCPPPEPPKENTDPYKNYKFWEVDLS
                     EKLSDQLDQYPLGRKFLNQSGLQRIGTKRPAPAPVSIVKSSKRKRRT"
     gene            6556..8073
                     /gene="L1"
                     /label="L1"
     terminator      8120..8166
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"