pBCGE7 vector (V009247)

Basic Vector Information

Vector Name:
pBCGE7
Antibiotic Resistance:
Kanamycin
Length:
6984 bp
Type:
Expression vector
Replication origin:
ori
Source/Author:
Govan VA, Williamson AL.

pBCGE7 vector Map

pBCGE76984 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900KanRoriThymidylate kinaseProtein E7rrnB T1 terminator

pBCGE7 vector Sequence

LOCUS       V009247                 6984 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009247
VERSION     V009247
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Govan VA, Williamson AL.
  TITLE     Rabbits immunised with recombinant BCG expressing the cottontail
            rabbit papillomavirus (CRPV) L2E7E2 genes induces regression of
            established papillomas
  JOURNAL   Virus Res. 127 (1), 43-48 (2007)
   PUBMED   17451831
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Govan VA, Williamson A-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2006) Institute of Infectious Disease and
            Molecular Medicine, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6984)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6984)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus
            Res."; date: "2007"; volume: "127"; issue: "1"; pages: "43-48"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-DEC-2006) Institute of Infectious Disease and Molecular
            Medicine, University of Cape Town, Anzio Road, Observatory, Cape
            Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1877..2689
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             5698..6330
                     /gene="tmk"
                     /label="Thymidylate kinase"
                     /note="Thymidylate kinase from Mycobacterium tuberculosis
                     (strain ATCC 25618 / H37Rv). Accession#: P9WKE1"
     CDS             6556..6837
                     /gene="E7"
                     /label="Protein E7"
                     /note="Protein E7 from Cottontail rabbit papillomavirus
                     (strain Kansas). Accession#: P03130"
     terminator      6902..6948
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"

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