Basic Vector Information
- Vector Name:
- pBCGE2
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7872 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Govan VA, Williamson AL.
pBCGE2 vector Map
pBCGE2 vector Sequence
LOCUS V009248 7872 bp DNA circular SYN 17-DEC-2018 DEFINITION Exported. ACCESSION V009248 VERSION V009248 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 7872) AUTHORS Govan VA, Williamson AL. TITLE Rabbits immunised with recombinant BCG expressing the cottontail rabbit papillomavirus (CRPV) L2E7E2 genes induces regression of established papillomas JOURNAL Virus Res. 127 (1), 43-48 (2007) PUBMED 17451831 REFERENCE 2 (bases 1 to 7872) AUTHORS Govan VA, Williamson A-L. TITLE Direct Submission JOURNAL Submitted (10-DEC-2006) Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa REFERENCE 3 (bases 1 to 7872) TITLE Direct Submission REFERENCE 4 (bases 1 to 7872) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res."; date: "2007"; volume: "127"; issue: "1"; pages: "43-48" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (10-DEC-2006) Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7872 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 1877..2689 /label="KanR" /note="aminoglycoside phosphotransferase" rep_origin 3024..3612 /direction=RIGHT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 5698..6330 /gene="tmk" /label="Thymidylate kinase" /note="Thymidylate kinase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). Accession#: P9WKE1" CDS 6556..7725 /gene="E2" /label="Regulatory protein E2" /note="Regulatory protein E2 from Cottontail rabbit papillomavirus (strain Kansas). Accession#: P03121" terminator 7790..7836 /label="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene"
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