Basic Vector Information
- Vector Name:
- pBAT
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9691 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kooij TW, Rauch MM, Matuschewski K.
pBAT vector Map
pBAT vector Sequence
LOCUS V009274 9691 bp DNA circular SYN 17-DEC-2018
DEFINITION Exported.
ACCESSION V009274
VERSION V009274
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9691)
AUTHORS Kooij TW, Rauch MM, Matuschewski K.
TITLE Expansion of experimental genetics approaches for Plasmodium berghei
with versatile transfection vectors
JOURNAL Mol. Biochem. Parasitol. 185 (1), 19-26 (2012)
PUBMED 22705315
REFERENCE 2 (bases 1 to 9691)
AUTHORS Kooij TWA.
TITLE Direct Submission
JOURNAL Submitted (23-MAY-2012) Parasitology Unit, Max Planck Institute for
Infection Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany
REFERENCE 3 (bases 1 to 9691)
AUTHORS Kooij TWA.
TITLE Direct Submission
JOURNAL Submitted (29-AUG-2012) Parasitology Unit, Max Planck Institute for
Infection Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany
REFERENCE 4 (bases 1 to 9691)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 9691)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
Biochem. Parasitol."; date: "2012"; volume: "185"; issue: "1";
pages: "19-26"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(23-MAY-2012) Parasitology Unit, Max Planck Institute for Infection
Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany"
SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(29-AUG-2012) Parasitology Unit, Max Planck Institute for Infection
Biology, Chariteplatz 1, Berlin, Berlin 10117, Germany"
SGRef: number: 4; type: "Journal Article"
On Aug 29, 2012 this sequence version replaced JX099571.1.
FEATURES Location/Qualifiers
source 1..9691
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 2..18
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 19..37
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 79..132
/note="5'MCS; multiple cloning site"
CDS 166..870
/label="mCherry"
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
misc_feature 904..933
/label="c-Myc epitope tag"
/note="c-Myc epitope tag"
CDS 904..933
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label="Myc"
/translation="EQKLISEEDL"
misc_feature 940..969
/label="c-Myc epitope tag"
/note="c-Myc epitope tag"
CDS 940..969
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label="Myc"
/translation="EQKLISEEDL"
misc_feature 976..1005
/label="c-Myc epitope tag"
/note="c-Myc epitope tag"
CDS 976..1005
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label="Myc"
/translation="EQKLISEEDL"
3'UTR 1021..1434
/note="3'PbPPPK-DHPS; 3'UTR of Plasmodium berghei
hydroxymethylpterin pyrophosphokinase-dihydropteroate
synthetase"
5'UTR 1456..2825
/note="5'PbHSP70; 5'UTR of Plasmodium berghei heat shock
protein 70"
CDS 2849..3562
/label="yeGFP"
/note="yeast-enhanced green fluorescent protein"
3'UTR 3578..4107
/note="3'PbDHFS-FPGS; 3'UTR of Plasmodium berghei
dihydrofolate synthase-folylpolyglutamate synthase"
3'UTR complement(4120..4575)
/note="3'PbDHFR-TS; 3'UTR of Plasmodium berghei
dihydrofolate reductase-thymidylate synthase"
CDS complement(4579..5223)
/gene="FUR1"
/label="Uracil phosphoribosyltransferase"
/note="Uracil phosphoribosyltransferase from Saccharomyces
cerevisiae (strain ATCC 204508 / S288c). Accession#:
P18562"
CDS complement(5224..5694)
/gene="FCY1"
/label="Cytosine deaminase"
/note="Cytosine deaminase from Saccharomyces cerevisiae
(strain ATCC 204508 / S288c). Accession#: Q12178"
CDS complement(5737..6297)
/gene="DHFR"
/label="Dihydrofolate reductase"
/note="Dihydrofolate reductase from Homo sapiens.
Accession#: P00374"
5'UTR complement(6298..7241)
/note="5'PbDHFR-TS; 5'UTR of Plasmodium berghei
dihydrofolate reductase-thymidylate synthase"
misc_feature complement(7248..7694)
/note="3'PbDHFR-TS_duplicate; duplicated sequence of 3'UTR
of Plasmodium berghei dihydrofolate reductase-thymidylate
synthase"
misc_feature 7695..7757
/note="3'MCS; multiple cloning site"
promoter complement(7797..7814)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7819..7835)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(7996..8584)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8758..9615)
/label="AmpR"
/note="beta-lactamase"
promoter complement(9616..9687)
/label="AmpR promoter"
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