pBAN vector (V009280)

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LOCUS       40924_5909        3781 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Yadong H, Chunfang X, Dongsheng Y.
  TITLE     Biobrick parts and vectors for Heterologous protein production using
            the Pichia pastoris
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Yadong H, Chunfang X, Dongsheng Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2013) Institute of Microbial Biotechnology, 
            Institute of Biomedicine, Jinan University, No. 601, Huangpu, 
            Guangzhou, Guangdong 510632, China
REFERENCE   3  (bases 1 to 3781)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2013) Institute of Microbial Biotechnology, Institute of 
            Biomedicine, Jinan University, No. 601, Huangpu, Guangzhou, 
            Guangdong 510632, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     Name: the biobrick plasmid backbone, pBAN vector.
FEATURES             Location/Qualifiers
     source          1..3781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..21
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     regulatory      22..890
                     /note="derived from Aspergillus Niger glucoamylase gene;
                     Tgla"
                     /regulatory_class="terminator"
     misc_feature    951..1258
                     /note="5' upstream sequence Aspergillus Niger glucoamylase 
                     promoter"
     promoter        1290..1394
                     /label=AmpR promoter
     CDS             1395..2252
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2426..3014
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3302..3323
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3338..3368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3376..3392
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3400..3416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3447..3758
                     /note="3' upstream sequence of Aspergillus Niger
                     glucoamylase promoter"
     misc_feature    3760..3781
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"