pBAMD1-4 vector (V009282)

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LOCUS       40924_5899        4730 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAMD1-4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI.
  TITLE     New transposon tools tailored for metabolic engineering of 
            gram-negative microbial cell factories
  JOURNAL   Front Bioeng Biotechnol 2, 46 (2014)
  PUBMED    25389526
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2014) Systems Biology Program, Centro Nacional de 
            Biotecnologia (CNB-CSIC), C/Darwin 3, Madrid, Madrid 28049, Spain
REFERENCE   3  (bases 1 to 4730)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4730)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol 2, 46 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-AUG-2014) Systems Biology Program, Centro Nacional de 
            Biotecnologia (CNB-CSIC), C/Darwin 3, Madrid, Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..28
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     misc_feature    62..118
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      127..156
                     /label=terminator T500
                     /note="terminator T500"
                     /regulatory_class="terminator"
     CDS             362..1150
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      1164..1235
                     /label=terminator of gene 32 (T32)
                     /note="terminator of gene 32 (T32)"
                     /regulatory_class="terminator"
     misc_feature    complement(1246..1264)
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     terminator      1275..1369
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1474..2901
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
     promoter        2958..3049
                     /label=AmpR promoter
     CDS             3050..3907
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      3914..3953
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            4101..4209
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4220..4608
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     terminator      complement(4639..4725)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"