pBADSce vector (V009287)

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LOCUS       V009287                 6722 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009287
VERSION     V009287
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Lopez CM, Rholl DA, Trunck LA, Schweizer HP.
  TITLE     Versatile dual-technology system for markerless allele replacement
            in Burkholderia pseudomallei
  JOURNAL   Appl. Environ. Microbiol. 75 (20), 6496-6503 (2009)
   PUBMED   19700544
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Lopez CM, Schweizer HP.
  TITLE     Homing Endonuclease-based System for Select Agent-compliant
            Markerless Gene Replacement in Burkholderia pseudomallei
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6722)
  AUTHORS   Lopez CM, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Microbiology, Immunology and Patholofy,
            Colorado State University, 3205 Rampart Rd., Fort Collins, CO 80523,
            USA
REFERENCE   4  (bases 1 to 6722)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6722)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2009"; volume: "75"; issue: "20";
            pages: "6496-6503"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2009) Microbiology, Immunology and Patholofy, Colorado State
            University, 3205 Rampart Rd., Fort Collins, CO 80523, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      216..302
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      394..421
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        440..531
                     /label="AmpR promoter"
     CDS             complement(933..1304)
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     promoter        complement(1323..1370)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     rep_origin      complement(1490..1841)
                     /direction=LEFT
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             1855..2685
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      2918..3373
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      3484..4072
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4772..5647)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        5674..5958
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             5999..6703
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"