pBAD24hyg vector (V009295)

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LOCUS       V009295                 4619 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009295
VERSION     V009295
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     MipZ, a spatial regulator coordinating chromosome segregation with
            cell division in Caulobacter
  JOURNAL   Cell 126 (1), 147-162 (2006)
   PUBMED   16839883
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2005) Developmental Biology, Stanford University,
            279 Campus Drive, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 4619)
  AUTHORS   Thanbichler M, Shapiro L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2006) Developmental Biology, Stanford University,
            279 Campus Drive, Stanford, CA 94305, USA
REFERENCE   4  (bases 1 to 4619)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4619)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell";
            date: "2006"; volume: "126"; issue: "1"; pages: "147-162"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-OCT-2005) Developmental Biology, Stanford University, 279 Campus
            Drive, Stanford, CA 94305, USA"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (18-JAN-2006) Developmental Biology, Stanford University, 279 Campus
            Drive, Stanford, CA 94305, USA"
            SGRef: number: 4; type: "Journal Article"
            On Jan 18, 2006 this sequence version replaced DQ266354.1.
FEATURES             Location/Qualifiers
     source          1..4619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     regulatory      1306..1312
                     /regulatory_class="ribosome_binding_site"
     terminator      1569..1655
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1747..1774
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1793..1884
                     /label="AmpR promoter"
     CDS             2058..3053
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     rep_origin      3430..4018
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"