pBAD24-rnc-sfGFP vector (V009298)

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LOCUS       V009298                 5932 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009298
VERSION     V009298
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Malagon F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2013) Laboratory of Molecular Biology, National
            Cancer Institute, Convent Drive, Building 37, Room 5138, Bethesda,
            MD 20892-4255, USA
REFERENCE   2  (bases 1 to 5932)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (07-MAY-2013) Laboratory of Molecular Biology, National Cancer
            Institute, Convent Drive, Building 37, Room 5138, Bethesda, MD
            20892-4255, USA"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             1321..1998
                     /gene="rnc"
                     /label="Ribonuclease 3"
                     /note="Ribonuclease 3 from Escherichia coli (strain K12 /
                     MC4100 / BW2952). Accession#: C4ZYJ0"
     CDS             2014..2724
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     terminator      2959..3045
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3137..3164
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3183..3274
                     /label="AmpR promoter"
     CDS             3275..4132
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4177..4632
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      4743..5331
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"