pBAD18-His6-hTERT-LPTS-L vector (V009301)

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LOCUS       V009301                 9120 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009301
VERSION     V009301
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Hansen DT, Thiyagarajan T, Larson AC, Hansen JL.
  TITLE     Telomerase repeat amplification protocol (TRAP) activity upon
            recombinant expression and purification of human telomerase in a
            bacterial system
  JOURNAL   Protein Expr. Purif. (2016) In press
   PUBMED   26965413
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Hansen DT, Hansen JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2015) Department of Biochemistry and Molecular
            Biology, Medical University of South Carolina, 173 Ashley Avenue,
            Charleston, SC 29425, USA
REFERENCE   3  (bases 1 to 9120)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein
            Expr. Purif. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-DEC-2015) Department of Biochemistry and Molecular Biology,
            Medical University of South Carolina, 173 Ashley Avenue, Charleston,
            SC 29425, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     RBS             1312..1334
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1353..1370
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1389..4784
                     /label="hTERT"
                     /note="human telomerase reverse transcriptase, the
                     catalytic subunit of telomerase"
     CDS             4868..5851
                     /gene="PINX1"
                     /label="PIN2/TERF1-interacting telomerase inhibitor 1"
                     /note="PIN2/TERF1-interacting telomerase inhibitor 1 from
                     Homo sapiens. Accession#: Q96BK5"
     terminator      6072..6158
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6250..6277
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        6296..6387
                     /label="AmpR promoter"
     CDS             6388..7245
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7290..7745
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      7856..8444
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8630..8770)
                     /label="bom"
                     /note="basis of mobility region from pBR322"