pBACVM4 vector (V009306)

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LOCUS       V009306                 9077 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009306
VERSION     V009306
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Schoenborn MA, Lippmann W, Amemiya CT.
  TITLE     BAC Cloning Plasmid: pBAC-VM4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Schoenborn MA, Lippmann W, Amemiya CT.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2003) Molecular Genetics, Benaroya Research
            Institute at Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101, USA
REFERENCE   3  (bases 1 to 9077)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-MAY-2003) Molecular Genetics, Benaroya Research Institute at
            Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(248..1663)
                     /gene="sacB"
                     /label="Levansucrase"
                     /note="Levansucrase from Bacillus amyloliquefaciens.
                     Accession#: P21130"
     promoter        1763..1781
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    1792..1848
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1891..1907)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1915..1931)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1939..1969)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1984..2005)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2228..2884)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2885..2987)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      3845..4459
                     /label="oriV"
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4535..4754
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             4845..5597
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     misc_feature    5603..5853
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6179..7351
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             7354..8322
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    8398..8871
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"