pBACr-AraT7940_rbs9 vector (V009309)

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LOCUS       40924_5689       10508 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBACr-AraT7940_rbs9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
  PUBMED    17700541
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control 
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
  PUBMED    19801975
REFERENCE   3  (bases 1 to 10508)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates 
            and chemical 'wires'
  JOURNAL   Nature 469 (7329), 212-215 (2011)
  PUBMED    21150903
REFERENCE   4  (bases 1 to 10508)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 10508)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 10508)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Syst. 
            Biol. 3, 133 (2007)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2009"; volume: "27"; issue: "10"; pages: 
            "946-950"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nature"; 
            date: "2011"; volume: "469"; issue: "7329"; pages: "212-215"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (06-JAN-2012) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(105..980)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        1007..1291
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      1302..1334
                     /label=RBS9
                     /note="RBS9"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1335..4001
                     /gene="T7ptag"
                     /label=similar to T7ptag amberstop
                     /note="similar to T7ptag amberstop"
     gene            1335..4001
                     /gene="T7ptag"
                     /label=T7ptag
     misc_feature    1350..1352
                     /gene="T7ptag"
                     /label=amberstop
                     /note="amberstop"
     misc_feature    1668..1670
                     /gene="T7ptag"
                     /label=amberstop
                     /note="amberstop"
     CDS             4021..4050
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             4066..4083
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      4309..4355
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(4411..4799)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(4852..5643)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6049..6268
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6359..7111
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    7117..7367
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             7693..8865
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             8868..9836
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     gene            9912..10429
                     /gene="parC"
                     /label=parC
     misc_feature    9912..10385
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"