pBacHTS vector (V009320)

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LOCUS       40924_5634        6205 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Baculovirus shuttle vector pBacHTS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Choi EW, Seen DS, Song YB, Son HS, Jung NC, Huh WK, Hahn JS, Kim K, 
            Jeong JY, Lee TG.
  TITLE     AdHTS: a high-throughput system for generating recombinant 
            adenoviruses
  JOURNAL   J. Biotechnol. 162 (2-3), 246-252 (2012)
  PUBMED    23063969
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Son H.
  TITLE     BacHTS: a high-throughput system for recombinant baculovirus 
            expression
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6205)
  AUTHORS   Son H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) Seoul National University, BRNSCIENCE CO., 
            Ltd., R
REFERENCE   4  (bases 1 to 6205)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 162 (2-3), 246-252 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2011) Seoul National University, BRNSCIENCE CO., Ltd., R"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1158..1249
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     protein_bind    1267..1391
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1534..1836
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1856..1980)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     2004..3361
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      3508..3963
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4245..4349
                     /label=AmpR promoter
     CDS             4350..5207
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5381..5969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"