pBac3xP3DsRed2attP vector (V009327)

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LOCUS       40924_5564        6495 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Accepter vector pBac3xP3DsRed2attP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Yonemura N, Tamura T, Uchino K, Kobayashi I, Tatematsu K, Iizuka T, 
            Sezutsu H, Muthulakshmi M, Nagaraju J, Kusakabe T.
  TITLE     PhiC31 integrase-mediated cassette exchange in silkworm embryos
  JOURNAL   Mol. Genet. Genomics 287 (9), 731-739 (2012)
  PUBMED    22842670
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Yonemura N, Kobayashi I, Uchino K, Sezutsu H, Tamura T, Kusakabe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2012) Contact:Naoyuki Yonemura National Institute 
            of Agrobiological Sciences (NIAS), MAFF; Ohwashi 1-2, Tsukuba 
            Science City 305-8634, Japan
REFERENCE   3  (bases 1 to 6495)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6495)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Genet.
            Genomics"; date: "2012"; volume: "287"; issue: "9"; pages: "731-739"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2012) Contact:Naoyuki Yonemura National Institute of 
            Agrobiological Sciences (NIAS), MAFF; Ohwashi 1-2, Tsukuba Science 
            City 305-8634, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    705..1473
                     /label=piggyBac right arm
                     /note="piggyBac right arm"
     misc_recomb     complement(1490..1528)
                     /label=Streptomyces phage phiC31 attP sequence
                     /note="Streptomyces phage phiC31 attP sequence"
     protein_bind    1535..1559
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1604..1879
                     /gene="3xP3"
                     /label=Artificial Pax6 responsive promoter
                     /note="Artificial Pax6 responsive promoter"
                     /regulatory_class="promoter"
     gene            1604..1879
                     /gene="3xP3"
                     /label=3xP3
     CDS             1880..2554
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     polyA_signal    2678..2812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2818..2842)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(2849..2887)
                     /label=Streptomyces phage phiC31 attP sequence
                     /note="Streptomyces phage phiC31 attP sequence"
     misc_feature    3018..3696
                     /label=piggyBac left arm
                     /note="piggyBac left arm"
     primer_bind     complement(3881..3897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3905..3921)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3929..3959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3974..3995)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4283..4871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5045..5902)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5903..6007)
                     /label=AmpR promoter