Basic Vector Information
- Vector Name:
- pBAC-SCenhanced
- Antibiotic Resistance:
- Tetracycline
- Length:
- 10494 bp
- Type:
- Cloning vector
- Replication origin:
- ori2
- Source/Author:
- Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA, Del Vecchio D, Collins JJ.
pBAC-SCenhanced vector Vector Map
pBAC-SCenhanced vector Sequence
LOCUS 40924_5544 10494 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pBAC-SCenhanced, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10494)
AUTHORS Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
Del Vecchio D, Collins JJ.
TITLE Creating Single-Copy Genetic Circuits
JOURNAL Mol. Cell 63 (2), 329-336 (2016)
PUBMED 27425413
REFERENCE 2 (bases 1 to 10494)
AUTHORS Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
Del Vecchio D, Collins JJ.
TITLE Direct Submission
JOURNAL Submitted (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St,
MIT Building E25-Rm302, Cambridge, MA 02142, USA
REFERENCE 3 (bases 1 to 10494)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10494)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell";
date: "2016"; volume: "63"; issue: "2"; pages: "329-336"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(18-MAY-2016) Biological Engineering, MIT, 45 Carleton St, MIT
Building E25-Rm302, Cambridge, MA 02142, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10494
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(105..1073)
/label=sopB
/note="partitioning protein for the bacterial F plasmid"
CDS complement(1076..2248)
/label=sopA
/note="partitioning protein for the bacterial F plasmid"
misc_feature complement(2574..2824)
/label=incC
/note="incompatibility region of the bacterial F plasmid"
CDS complement(2830..3582)
/label=repE
/note="replication initiation protein for the bacterial F
plasmid"
rep_origin complement(3673..3892)
/direction=LEFT
/label=ori2
/note="secondary origin of replication for the bacterial F
plasmid; also known as oriS"
rep_origin complement(3968..4582)
/direction=LEFT
/label=oriV
/note="origin of replication for the bacterial F plasmid"
promoter 5440..5542
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 5543..6199
/label=CmR
/note="chloramphenicol acetyltransferase"
terminator complement(6455..6549)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(6612..7325)
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
regulatory complement(7326..7349)
/label=G5
/note="G5"
/regulatory_class="ribosome_binding_site"
RBS 7331..7339
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
regulatory complement(7367..7440)
/label=PLtetO
/note="PLtetO"
/regulatory_class="promoter"
promoter complement(7367..7440)
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
prim_transcript 7386
/label=transcription start
/note="transcription start"
protein_bind 7397..7415
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 7422..7440
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS complement(7486..8565)
/label=lacI
/note="lac repressor"
promoter complement(8602..8675)
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
promoter 8707..8736
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
prim_transcript 8742
/label=transcription start
/note="transcription start"
protein_bind 8744..8760
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 8807..9427
/label=TetR
/note="tetracycline repressor TetR"
regulatory 9473..9547
/label=Ptrc-2
/note="Ptrc-2"
/regulatory_class="promoter"
promoter 9486..9515
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 9523..9539
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
prim_transcript 9523
/label=transcription start
/note="transcription start"
regulatory 9555..9581
/label=M2
/note="M2"
/regulatory_class="ribosome_binding_site"
RBS 9568..9576
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 9582..10289
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
terminator 10352..10438
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
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