pBac-IE2-HsCBDBMP4-Puro vector (V009338)

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LOCUS       40924_5509        6062 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBac-IE2-HsCBDBMP4-Puro DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Imai S, Li Z, Miyagawa Y, Toyoda M, Umezawa A, Mon H, Kusakabe T, 
            Yoshimura K, Lee JM.
  TITLE     Biologically active human bone morphogenetic protein 4-fused to 
            collagen binding domain produced in silkworm-baculovirus expression 
            system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Lee JM, Li Z, Kusakabe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2013) Contact:Jae Man Lee Laboratory of Silkworm 
            Science, Kyushu University Graduate School of Bioresource and 
            Bioenvironmental Sciences; 6-10-1 Hakozaki, Higashi-ku, Fukuoka 
            812-8581, Japan E-mail :jaemanle@agr.kyushu-u.ac.jp Phone 
            :81-92-642-2842 Fax :81-92-642-2842
REFERENCE   3  (bases 1 to 6062)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2013) Contact:Jae Man Lee Laboratory of Silkworm Science, 
            Kyushu University Graduate School of Bioresource and 
            Bioenvironmental Sciences"; volume: " 6-10-1 Hakozaki, Higashi-ku, 
            Fukuoka 812-8581, Japan E-mail :jaemanle@agr.kyushu-u.ac.jp Phone 
            :81-92-642-2842 Fax "; pages: "81-92-642-284"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        371..662
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     CDS             684..1280
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    1484..1618
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1633..2180
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     protein_bind    2215..2235
                     /label=attB1
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     sig_peptide     2259..2321
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     CDS             3006..3020
                     /codon_start=1
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
                     /translation="DDDDK"
     CDS             3405..3425
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             3435..3458
                     /codon_start=1
                     /label=8xHis
                     /note="8xHis affinity tag"
                     /translation="HHHHHHHH"
     protein_bind    complement(3462..3486)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3516..3557
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             3567..3584
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    3602..3731
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     promoter        4278..4382
                     /label=AmpR promoter
     CDS             4383..5240
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5414..6002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"