pBAC-DE vector (V009341)

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LOCUS       V009341                 8821 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009341
VERSION     V009341
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Na G, Wolfe A, Ko C, Youn H, Lee YM, Byun SJ, Jeon I, Koo Y.
  TITLE     A Low-Copy-Number Plasmid for Retrieval of Toxic Genes from BACs and
            Generation of Conditional Targeting Constructs
  JOURNAL   Mol. Biotechnol. 54 (2), 504-514 (2013)
   PUBMED   22945876
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Koo Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2010) Biological Sciences, Inje University, 607
            Obangdong, Gimae, Gyongnam 621-749, South Korea
REFERENCE   3  (bases 1 to 8821)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
            Biotechnol."; date: "2013"; volume: "54"; issue: "2"; pages:
            "504-514"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-NOV-2010) Biological Sciences, Inje University, 607 Obangdong,
            Gimae, Gyongnam 621-749, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..27
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(57..75)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             175..1590
                     /gene="sacB"
                     /label="Levansucrase"
                     /note="Levansucrase from Bacillus amyloliquefaciens.
                     Accession#: P21130"
     promoter        complement(2015..2043)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     protein_bind    2072..2105
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    complement(2375..2848)
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(2924..3892)
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(3895..5067)
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(5393..5643)
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(5649..6401)
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     rep_origin      complement(6492..6711)
                     /direction=LEFT
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     promoter        7638..7740
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             7741..8397
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(8649..8682)
                     /label="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
                     compatible with each other, but incompatible with wild-type
                     loxP sites."