Basic Vector Information
- Vector Name:
- pB4cCGGW
- Antibiotic Resistance:
- Streptomycin
- Length:
- 11904 bp
- Type:
- Gateway vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Kamigaki A, Nito K, Hikino K, Goto-Yamada S, Nishimura M, Nakagawa T, Mano S.
- Promoter:
- CaMV35S(long)
pB4cCGGW vector Vector Map
pB4cCGGW vector Sequence
LOCUS 40924_5239 11904 bp DNA circular SYN 17-DEC-2018
DEFINITION Gateway vector pB4cCGGW DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11904)
AUTHORS Kamigaki A, Nito K, Hikino K, Goto-Yamada S, Nishimura M, Nakagawa
T, Mano S.
TITLE Gateway Vectors for Simultaneous Detection of Multiple
Protein-Protein Interactions in Plant Cells Using Bimolecular
Fluorescence Complementation
JOURNAL PLoS ONE 11 (8), E0160717 (2016)
PUBMED 27490375
REFERENCE 2 (bases 1 to 11904)
AUTHORS Mano S, Nakagawa T.
TITLE Direct Submission
JOURNAL Submitted (10-JUL-2013) Contact:Shoji Mano National Institute for
Basic Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji,
Okazaki, Aichi 444-8585, Japan
REFERENCE 3 (bases 1 to 11904)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11904)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2016"; volume: "11"; issue: "8"; pages: "E0160717"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-JUL-2013) Contact:Shoji Mano National Institute for Basic
Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji,
Okazaki, Aichi 444-8585, Japan"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT These colnes were obtained at our laboratory.
FEATURES Location/Qualifiers
source 1..11904
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(114..138)
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 341..357
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 872..1217
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 1238..1435
/label=cecfp
/note="cecfp"
protein_bind 1445..1569
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 1622..1652
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 1706..2362
/codon_start=1
/label=CmR
/note="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHSCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 2707..3009
/codon_start=1
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(3053..3177)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
terminator 3203..3455
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(3489..3505)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3513..3529
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3537..3567)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3582..3603)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
terminator complement(3637..3889)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(4282..5070)
/codon_start=1
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase from Tn5"
/translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(5094..5277)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature complement(5483..5507)
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 6035..6823
/codon_start=1
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 7070..7658
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(7844..7984)
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(8328..8522)
/direction=LEFT
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(8591..9655)
/codon_start=1
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
CDS complement(10092..10718)
/codon_start=1
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
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