pB1H1 vector (V009374)

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LOCUS       40924_5214        4485 bp DNA     circular SYN 17-DEC-2018
DEFINITION  TF expression vector pB1H1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Meng X, Brodsky MH, Wolfe SA.
  TITLE     A bacterial one-hybrid system for determining the DNA-binding 
            specificity of transcription factors
  JOURNAL   Nat. Biotechnol. 23 (8), 988-994 (2005)
  PUBMED    16041365
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Meng X, Brodsky MH, Wolfe SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2006) Program in Gene Function and Expression, 
            University of Massachusetts Medical School, 364 Plantation St., 
            Worcester, MA 01605, USA
REFERENCE   3  (bases 1 to 4485)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2005"; volume: "23"; issue: "8"; pages: 
            "988-994"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-APR-2006) Program in Gene Function and Expression, University of
            Massachusetts Medical School, 364 Plantation St., Worcester, MA 
            01605, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1842..1872
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    1880..1896
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1904..1920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2719..2742
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     CDS             complement(join(4048..4485,1..219))
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"