Basic Vector Information
- Vector Name:
- pB-UGateway w+
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11005 bp
- Type:
- PiggyBac transformation vector
- Replication origin:
- ori
- Source/Author:
- Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
- Promoter:
- hsp70
pB-UGateway w+ vector Vector Map
pB-UGateway w+ vector Sequence
LOCUS 40924_5204 11005 bp DNA circular SYN 17-DEC-2018
DEFINITION PiggyBac transformation vector pB-UGateway w+, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11005)
AUTHORS Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
TITLE A toolkit for transformation and mutagenesis in Drosophila using
piggyBac
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11005)
AUTHORS Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2002) Invertebrate Targets, Syngenta
Biotechnology, Inc., 3054 Cornwallis Rd, Research Triangle Park, NC
27709, USA
REFERENCE 3 (bases 1 to 11005)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11005)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-DEC-2002) Invertebrate Targets, Syngenta Biotechnology, Inc.,
3054 Cornwallis Rd, Research Triangle Park, NC 27709, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..11005
/mol_type="other DNA"
/organism="synthetic DNA construct"
mobile_element complement(11..620)
/mobile_element_type="transposon:piggyBac
transposableelement"
/label=ent
protein_bind 646..740
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 759..997
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
protein_bind 1006..1130
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 1155..1185
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 1239..1895
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 2240..2542
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(2586..2710)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
intron 2726..3040
/number=3
/note="RpS5"
protein_bind complement(3089..3113)
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 3271..3404
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3441..3457)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3465..3481
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3489..3519)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3534..3555
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
gene 3579..7698
/label=mini-white
/note="This modified version of the white gene lacks part
of the first intron."
mobile_element complement(7698..8147)
/mobile_element_type="transposon:piggyBac
transposableelement"
/label=ent
promoter complement(8165..8183)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(8204..8220)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 8228..8244
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8252..8282)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(8297..8318)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(8606..9194)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9368..10225)
/label=AmpR
/note="beta-lactamase"
promoter complement(10226..10330)
/label=AmpR promoter
rep_origin complement(10356..10811)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 10953..10969
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 10979..10997
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
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