pB-MCS w+ vector (V009380)

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LOCUS       40924_5179        8137 bp DNA     circular SYN 17-DEC-2018
DEFINITION  PiggyBac transformation vector pB-MCS w+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     A toolkit for transformation and mutagenesis in Drosophila using 
            piggyBac
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2002) Invertebrate Targets, Syngenta 
            Biotechnology, Inc., 3054 Cornwallis Rd, Research Triangle Park, NC 
            27709, USA
REFERENCE   3  (bases 1 to 8137)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8137)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2002) Invertebrate Targets, Syngenta Biotechnology, Inc., 
            3054 Cornwallis Rd, Research Triangle Park, NC 27709, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(11..625)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     primer_bind     642..658
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(692..708)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            711..4830
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     mobile_element  complement(4836..5284)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     promoter        complement(5297..5315)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5336..5352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5360..5376)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5384..5414)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5429..5450)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5738..6326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6500..7357)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7358..7462)
                     /label=AmpR promoter
     rep_origin      complement(7488..7943)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8085..8101
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8111..8129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"