pAYE vector (V009383)

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LOCUS       40924_5159        4603 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Phagemid vector pAYE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Yee A, Tan F-L., Ginsburg D.
  TITLE     Functional display of platelet-binding VWF fragments on filamentous 
            bacteriophage
  JOURNAL   PLoS ONE 8 (9), E73518 (2013)
  PUBMED    24019925
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Yee A, Tan F-L., Ginsburg D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2013) Life Scieneces Institute, University of 
            Michigan, 210 Washtenaw Ave, Ann Arbor, MI 48109, USA
REFERENCE   3  (bases 1 to 4603)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4603)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "9"; pages: "E73518"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUL-2013) Life Scieneces Institute, University of Michigan, 210 
            Washtenaw Ave, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2269..3723
                     /codon_start=1
                     /transl_except="(pos: 2326 .. 2328,  aa: OTHER)"
                     /product="VWF A1-pIII fusion protein"
                     /label=VWF A1-pIII fusion protein
                     /note="stop codon is suppressed in Eshcerichia coli
                     containing the glnV mutation (supE)"
                     /protein_id="AHB62418.1"
                     /translation="MRVLLFLLLSLFMLPAFSA*PAGAPGGQEPGGLVVPPTDAPVSPT
                     TLYVEDISEPPLHDFYCSRLLDLVFLLDGSSRLSEAEFEVLKAFVVDMMERLRISQKWV
                     RVAVVEYHDGSHAYIGLKDRKRPSELRRIASQVKYAGSQVASTSEVLKYTLFQIFSKID
                     RPEASRIALLLMASQEPQRMSRNFVRYVQGLKKKKVIVIPVGIGPHANLKQIRLIEKQA
                     PENKAFVLSSVDELEQQRDEIVSYLCDLAPEAPPPTLPPDMAQVGGGRAAAAGAPVPYP
                     DPLEPRAAQGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEK
                     MANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAG
                     SNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGV
                     FAFLLYVATFMYVFSTFANILRNKES"
     sig_peptide     2269..3720
     misc_feature    2326..2328
                     /label=amber stop
                     /note="amber stop"
     primer_bind     complement(3733..3749)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3962..4417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"