pAXT vector (V009386)

LOCUS       V009386                10639 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009386
VERSION     V009386
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Lu Y, Stegemann S, Agrawal S, Karcher D, Ruf S, Bock R.
  TITLE     Horizontal Transfer of a Synthetic Metabolic Pathway between Plant
            Species
  JOURNAL   Curr. Biol. (2017) In press
   PUBMED   28943084
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   Karcher D, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2017) Organelle Biology, Biotechnology and
            Molecular Ecophysiology, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg 1, Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 10639)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10639)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. Biol.
            (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-AUG-2017) Organelle Biology, Biotechnology and Molecular
            Ecophysiology, Max Planck Institute of Molecular Plant Physiology,
            Am Muehlenberg 1, Potsdam, Brandenburg 14476, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..54
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      73..201
                     /label="Prrn"
                     /note="Prrn"
                     /regulatory_class="promoter"
     5'UTR           202..243
     CDS             244..1758
                     /codon_start=1
                     /gene="Lyc"
                     /product="lycopene beta-cyclase"
                     /label="Lyc"
                     /note="derived from daffodil"
                     /protein_id="ATG32096.1"
                     /translation="MDTLLRTHNRLELLYPLHELAKRHFLSPSPNPQNPNFKFFSRKPY
                     QKKCRNGYIGVSSNQLLDLVPEIKKEHLEFDLPLYDPSKALTLDLAVVGGGPAGLAVAQ
                     QVSEAGLSVVSIDPNPKLIWPNNYGVWVDEFEDMDLLDCLDATWSGAIVYVDDRSTKNL
                     SRPYARVNRKNLKSKMMKKCVSNGVRFHQATVVKAMHEEEKSYLICSDGVTIDARVVLD
                     ATGFSRCLVQYDKPYNPGYQVAYGILAEVEEHPFDVDKMVFMDWRDSHLNGKAELNERN
                     AKIPTFLYAMPFSSNRIFLEETSLVARPGLKMEDIQERMVARLNHLGIRIKSIEEDERC
                     VIPMGGPLPVIPQRVVGIGGTAGMVHPSTGYMVARTLAAAPIVANSIVQYLVSDSGLSG
                     NDLSADVWKDLWPIERRRQREFFCFGMDILLKLDLEGTRRFFDAFFDLEPRYWHGFLSS
                     RLFLPELVPFGLSLFSHASNTCKLEIMAKGTLPLVNMINNLVQDRD"
     gene            244..1758
                     /gene="Lyc"
                     /label="Lyc"
                     /note="NpLyc"
     regulatory      1769..1918
                     /label="derived from Nicotiana tabacum rps16"
                     /note="derived from Nicotiana tabacum rps16"
                     /regulatory_class="terminator"
     misc_feature    1931..1980
                     /note="IEE; intercistronic expression element"
     CDS             2005..2739
                     /codon_start=1
                     /gene="crtW"
                     /product="beta-carotene ketolase"
                     /label="crtW"
                     /note="derived from marine bacterium Brevundimonas"
                     /protein_id="ATG32097.1"
                     /translation="MTAAVAEPRIVPRQTWIGLTLAGMIVAGWGSLHVYGVYFHRWGTS
                     SLVIVPAIVAVQTWLSVGLFIVAHDAMHGSLAPGRPRLNAAVGRLTLGLYAGFRFDRLK
                     TAHHAHHAAPGTADDPDFYAPAPRAFLPWFLNFFRTYFGWREMAVLTALVLIALFGLGA
                     RPANLLTFWAAPALLSALQLFTFGTWLPHRHTDQPFADAHHARSSGYGPVLSLLTCFHF
                     GRHHEHHLTPWRPWWRLWRGES"
     gene            2005..2739
                     /gene="crtW"
                     /label="crtW"
                     /note="BsCrtW"
     regulatory      2746..2970
                     /label="derived from Nicotiana tabacum rbcL"
                     /note="derived from Nicotiana tabacum rbcL"
                     /regulatory_class="terminator"
     misc_feature    2976..3025
                     /note="IEE; intercistronic expression element"
     CDS             3045..3530
                     /codon_start=1
                     /gene="crtZ"
                     /product="beta-carotene hydroxylase"
                     /label="crtZ"
                     /note="derived from marine bacterium Brevundimonas"
                     /protein_id="ATG32098.1"
                     /translation="MAWLTWIALFLTAFLGMEAFAWIMHRYVMHGFLWSWHRSHHEPHD
                     HPLEKNDLFAVVFAAPAIVMVAVGLHLWPWALPVGLGITAYGMVYFFFHDGLVHRRFPT
                     GFSGRSGFWTRRIQAHRLHHAVRTREGCVSFGFLWVRSARALKAELAQKRGSSSSGA"
     gene            3045..3530
                     /gene="crtZ"
                     /label="crtZ"
                     /note="BsCrtZ"
     regulatory      3537..3758
                     /label="derived from Chlamydomonas rbcL"
                     /note="derived from Chlamydomonas rbcL"
                     /regulatory_class="terminator"
     misc_feature    complement(3792..3825)
                     /label="loxP"
                     /note="loxP"
     protein_bind    3792..3825
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     regulatory      complement(3826..4233)
                     /label="derived from Nicotiana tabacum psbA"
                     /note="derived from Nicotiana tabacum psbA"
                     /regulatory_class="terminator"
     CDS             complement(4237..5025)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      complement(5026..5200)
                     /note="Prrn; derived from Nicotiana tabacum"
                     /regulatory_class="promoter"
     protein_bind    complement(5201..5234)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     tRNA            complement(5427..5497)
                     /gene="trnG"
                     /product="tRNA-Gly"
                     /label="trnG"
     gene            complement(5427..5497)
                     /gene="trnG"
                     /label="trnG"
     misc_feature    complement(5773..5903)
                     /label="similar to psbZ"
                     /note="similar to psbZ"
     promoter        complement(5917..5935)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5956..5972)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    5980..5996
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6004..6034)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6049..6070)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6358..6946)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7120..7977)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7978..8082)
                     /label="AmpR promoter"
     rep_origin      complement(8108..8563)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8705..8721
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8731..8749
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8761..9961
                     /label="similar to psaB"
                     /note="similar to psaB"
     CDS             10084..10383
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from Nicotiana
                     sylvestris. Accession#: Q3C1I0"
     tRNA            10536..10609
                     /gene="trnfM"
                     /product="tRNA-fMet"
                     /label="trnfM"
     gene            10536..10609
                     /gene="trnfM"
                     /label="trnfM"