pAVA319 vector (V009389)

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LOCUS       40924_5099        4780 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAVA319, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   von Arnim AG, Deng XW, Stacey MG.
  TITLE     Cloning vectors for the expression of green fluorescent protein 
            fusion proteins in transgenic plants
  JOURNAL   Gene 221 (1), 35-43 (1998)
  PUBMED    9852947
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   von Arnim AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUL-1998) Botany, The University of Tennessee, HBB437,
            Knoxville, TN 37996-1100, USA
REFERENCE   3  (bases 1 to 4780)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4780)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1998"; volume: "221"; issue: "1"; pages: "35-43"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-JUL-1998) Botany, The University of Tennessee, HBB437, 
            Knoxville, TN 37996-1100, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        789..1460
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1460..1601
                     /note="translational enhancer; 5'UTR from tobacco etch
                     virus"
     CDS             1615..2313
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     regulatory      2323..2533
                     /label=cauliflower mosaic virus 35S terminator
                     /note="cauliflower mosaic virus 35S terminator"
                     /regulatory_class="terminator"
     misc_feature    2534..2576
                     /label=polylinker
                     /note="polylinker"
     promoter        complement(2595..2613)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2634..2650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2658..2674)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2682..2712)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2727..2748)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3036..3624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3798..4655)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4656..4760)
                     /label=AmpR promoter