pAV012 vector (V009391)

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LOCUS       40924_5089        4577 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAV012, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   White KP, Victorsen A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2013) Human Genetics, The University of Chicago, 
            5801 South Ellis Avenue, Chicago, IL 60637, USA
REFERENCE   2  (bases 1 to 4577)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4577)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2013) Human Genetics, The University of Chicago, 5801 South 
            Ellis Avenue, Chicago, IL 60637, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      26..414
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     promoter        509..613
                     /label=AmpR promoter
     CDS             614..1471
                     /label=AmpR
                     /note="beta-lactamase"
     misc_feature    1583..1867
                     /note="sGFP exon 1; 1st exon of superfolder green
                     fluorescent protein"
     primer_bind     1583..1604
                     /label=forward recombineering primer binding site
                     /note="forward recombineering primer binding site"
     misc_feature    1941..1974
                     /label=loxP
                     /note="loxP"
     protein_bind    1941..1974
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        1989..2488
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        2505..2552
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2571..3371
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     protein_bind    complement(3507..3540)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3604..3622
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             4109..4126
                     /label=tetracysteine tag
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             4148..4171
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             4172..4192
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             4235..4258
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     primer_bind     complement(4265..4288)
                     /label=reverse recombineering primer binding site
                     /note="reverse recombineering primer binding site"
     oriT            complement(4334..4443)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"