pAT224 vector (V009413)

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LOCUS       40924_4974        5189 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pAT224, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter 
            MG, Pluckthun A.
  TITLE     High-affinity binders selected from designed ankyrin repeat protein 
            libraries
  JOURNAL   Nat. Biotechnol. 22 (5), 575-582 (2004)
  PUBMED    15097997
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Stumpp MT, Forrer P, Binz KH, Amstutz P, Zahnd C, Pluckthun A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2003) Biochemistry, University of Zurich, 
            Winterthurerstrasse 190, Zurich 8057, Switzerland
REFERENCE   3  (bases 1 to 5189)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2004"; volume: "22"; issue: "5"; pages: 
            "575-582"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2003) Biochemistry, University of Zurich, 
            Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             121..165
                     /label=AviTag(TM)
                     /note="peptide tag that allows for enzymatic biotinylation"
     CDS             178..1275
                     /label=MBP
                     /note="maltose binding protein from E. coli"
     misc_feature    1276..1317
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             1318..1335
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1352..1446
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      1553..1639
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1702..2781)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(2782..2859)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    3039..3179
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3365..3953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4127..4984)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4985..5089)
                     /label=AmpR promoter