pASRR vector (V009419)

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LOCUS       V009419                 5732 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009419
VERSION     V009419
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Park W-J., Han S-S., Kim H-S., Kim G-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2014) Interdisciplinary Graduate Program in
            Molecular Medicine, Chonnam National University, 77 Yongbong-ro,
            Buk-gu, Gwangju, Jeollanam-Do 500-757, Korea
REFERENCE   2  (bases 1 to 5732)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5732)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (12-AUG-2014) Interdisciplinary Graduate Program in Molecular
            Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu,
            Gwangju, Jeollanam-Do 500-757, Korea"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..201
                     /label="BEM promoter"
                     /note="BEM promoter"
                     /regulatory_class="promoter"
     CDS             210..959
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     terminator      1030..1116
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(1210..1237)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(1329..1415)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     1764..1780
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1793..2497)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    complement(2504..2591)
                     /label="derived from SodB"
                     /note="derived from SodB"
     protein_bind    complement(2611..2627)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      2679..2831
                     /label="luxI promoter"
                     /note="luxI promoter"
                     /regulatory_class="promoter"
     ncRNA           2838..2907
                     /product="RyhB"
                     /label="antisense_RNA"
                     /ncRNA_class="antisense_RNA"
     terminator      complement(2942..2985)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        3144..3246
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3247..3903
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     rep_origin      4640..5228
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5414..5554)
                     /label="bom"
                     /note="basis of mobility region from pBR322"