pASHSUL vector (V009420)

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LOCUS       40924_4924        3473 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pASHSUL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Weeks SD, Drinker M, Loll PJ.
  TITLE     Ligation independent cloning vectors for expression of SUMO fusions
  JOURNAL   Protein Expr. Purif. 53 (1), 40-50 (2007)
  PUBMED    17251035
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Weeks SD, Drinker M, Loll PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2007) Biochemistry and Molecular Biology, Drexel 
            University College of Medicine, 245 N 15th Street, Mailstop 497, 
            Philadelphia, PA 19102, USA
REFERENCE   3  (bases 1 to 3473)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3473)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "1"; pages: 
            "40-50"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2007) Biochemistry and Molecular Biology, Drexel University 
            College of Medicine, 245 N 15th Street, Mailstop 497, Philadelphia, 
            PA 19102, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..91
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             99..122
                     /codon_start=1
                     /product="TetA fragment"
                     /label=TetA fragment
                     /protein_id="ABN58907.1"
                     /translation="MNSSTKI"
     RBS             130..152
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             165..182
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             189..479
                     /label=SUMO
                     /note="cleavable ubiquitin-like protein tag"
     regulatory      505..576
                     /label=lpp terminator region
                     /note="lpp terminator region"
                     /regulatory_class="terminator"
     rep_origin      584..1039
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1066..1170
                     /label=AmpR promoter
     CDS             1171..2028
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             2038..2661
                     /label=TetR
                     /note="tetracycline repressor TetR"
     rep_origin      2817..3405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"