pASD2 vector (V009421)

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LOCUS       V009421                 5758 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009421
VERSION     V009421
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Day WA Jr., Rasmussen SL, Carpenter BM, Peterson SN, Friedlander AM.
  TITLE     Microarray analysis of transposon insertion mutations in Bacillus
            anthracis: global identification of genes required for sporulation
            and germination
  JOURNAL   J. Bacteriol. 189 (8), 3296-3301 (2007)
   PUBMED   17277068
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Day WA Jr., Rasmussen SL, Carpenter BM, Peterson SN, Friedlander AM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2006) Bacteriology Division, USAMRIID, 1425 Porter
            St, Frederick, MD 21702, USA
REFERENCE   3  (bases 1 to 5758)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5758)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2007"; volume: "189"; issue: "8"; pages:
            "3296-3301"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-AUG-2006) Bacteriology Division, USAMRIID, 1425 Porter St,
            Frederick, MD 21702, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..24
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     57..73
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             748..1539
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(1879..1895)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(1925..1943)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1979..3484
                     /label="pE194 temperature-sensitive replicon"
                     /note="pE194 temperature-sensitive replicon"
     rep_origin      complement(3667..4255)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4571..5350)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"