pASapI vector (V009423)

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LOCUS       V009423                 6623 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009423
VERSION     V009423
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Purton S.
  TITLE     pASapI: an expression vector for the Chlamydomonas chloroplast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Purton S.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6623)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6623)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-AUG-2013) Structural "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..25)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        499..603
                     /label="AmpR promoter"
     CDS             604..1461
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1635..2223
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    3200..3798
                     /gene="atpA"
                     /label="promoter and 5'UTR"
                     /note="promoter and 5'UTR"
     gene            3200..3798
                     /gene="atpA"
                     /label="atpA"
     3'UTR           3851..4257
                     /gene="rbcL"
     gene            3851..4257
                     /gene="rbcL"
                     /label="rbcL"
     CDS             complement(4413..4676)
                     /gene="psbH"
                     /label="Photosystem II reaction center protein H"
                     /note="Photosystem II reaction center protein H from
                     Chlamydomonas reinhardtii. Accession#: P22666"