pAS2.1-MLT3a vector (V009436)

LOCUS       V009436                10338 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009436
VERSION     V009436
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10338)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10338)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(965..1012)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(1060..1164)
                     /label="FLP terminator"
                     /note="FLP terminator"
                     /regulatory_class="terminator"
     promoter        1603..1883
                     /label="TRP1 promoter"
     CDS             1884..2555
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     rep_origin      complement(2661..3116)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3261..3277
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      complement(3314..3561)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(3858..4304)
                     /gene="RPL28"
                     /label="Large ribosomal subunit protein uL15"
                     /note="Large ribosomal subunit protein uL15 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P02406"
     promoter        4769..5473
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5501..5941
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             5993..6610
                     /codon_start=1
                     /note="unnamed protein product; hMLT fragment"
                     /protein_id="SJL86962.1"
                     /translation="VEIIIGRTDEAVECTEDELNNLGHPDNKEQTTDQPLAKDKVALLI
                     GNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVY
                     GLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDMCRK
                     RNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLANG"
     misc_feature    6569..6571
                     /label="stability creator"
                     /note="stability creator"
     regulatory      6638..6739
                     /label="ADH1 terminator"
                     /note="ADH1 terminator"
                     /regulatory_class="terminator"
     CDS             complement(6806..8014)
                     /codon_start=1
                     /note="unnamed protein product; IS10R"
                     /protein_id="SJL86963.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQ
                     KRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQL
                     VNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQ
                     KQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYAL
                     GKL"
     primer_bind     complement(8187..8203)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(8192..8229)
                     /label="5' UTR of lacZ, incl. RBS"
                     /note="5' UTR of lacZ, incl. RBS"
     protein_bind    8211..8227
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8235..8265)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8280..8301)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8589..9177)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9351..10208)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10209..10313)
                     /label="AmpR promoter"