pARAn58 vector (V009461)

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LOCUS       40924_4714        6144 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pARAn58, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Roth AH, Dersch P.
  TITLE     A novel expression system for intracellular production and 
            purification of recombinant affinity-tagged proteins in Aspergillus 
            niger
  JOURNAL   Appl. Microbiol. Biotechnol. 86 (2), 659-670 (2010)
  PUBMED    19908039
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Dersch P, Roth A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2009) Institute of Microbiology, Technische 
            Universitat Braunschweig, Spielmannstr. 7, Braunschweig 38106, 
            Germany
REFERENCE   3  (bases 1 to 6144)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6144)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2010"; volume: "86"; issue: "2"; 
            pages: "659-670"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAR-2009) Institute of Microbiology, Technische Universitat 
            Braunschweig, Spielmannstr. 7, Braunschweig 38106, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1194
                     /label=suc1
                     /note="suc1"
                     /regulatory_class="promoter"
     regulatory      1245..2313
                     /label=gla
                     /note="gla"
                     /regulatory_class="terminator"
     primer_bind     complement(2345..2361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2831..2935
                     /label=AmpR promoter
     CDS             2936..3793
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3967..4555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             join(5217..5373,5433..6100)
                     /codon_start=1
                     /gene="pyrG"
                     /product="orotidine-5'-phosphate decarboxylase"
                     /label=pyrG
                     /protein_id="ACP41233.1"
                     /translation="MSSKSHLPYAIRATNHPNPLTSKLFSIAEEKKTNVTVSADVTTSA
                     ELLDLADRLGPYIAVLKTHIDILTDLTPSTLSSLQSLATKHNFLIFEDRKFIDIGNTVQ
                     KQYHGGALRISEWAHIINCAILPGEGIVEALAQTTKSPDFKDANQRGLLILAEMTSKGS
                     LATGEYTARSVEYARKYKGFVMGFVSTRALSEVLPEQKEESEDFVVFTTGVNLSDKGDK
                     LGQQYQTPGSAVGRGADFIIAGRGIYKADDPVEAVQRYREEGWKAYEKRVGL"
     gene            5217..6100
                     /gene="pyrG"
                     /label=pyrG