pAP322 vector (V009472)

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LOCUS       V009472                 6491 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009472
VERSION     V009472
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Park AS, Rubin EJ, Minch KJ, Sherman DR.
  TITLE     Clp-mediated Regulation of WhiB1 is Required for Cell Division in
            Mycobacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Park AS, Rubin EJ, Minch KJ, Sherman DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2017) Department of Immunology and Infectious
            Diseases, Harvard School of Public Health, 665 Huntington Ave,
            Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 6491)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6491)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-JUL-2017) Department of Immunology and Infectious Diseases,
            Harvard School of Public Health, 665 Huntington Ave, Boston, MA
            02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..38
                     /regulatory_class="terminator"
     CDS             complement(73..693)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      complement(720..1036)
                     /regulatory_class="promoter"
     terminator      complement(1055..1082)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(1264..1852)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2187..2999)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(3283..4395)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     terminator      complement(4942..4988)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5034..5061
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      5086..5391
                     /regulatory_class="promoter"
     protein_bind    5261..5278
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      5407..5412
                     /regulatory_class="ribosome_binding_site"
     CDS             5438..5455
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             5468..6181
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             6191..6442
                     /codon_start=1
                     /product="WhiB1"
                     /label="WhiB1"
                     /note="WhiB1 from Mycobacterium smegmatis str. MC2 155"
                     /protein_id="ASU06035.1"
                     /translation="DWRHKAVCRDEDPELFFPVGNSGPALAQIADAKLVCNRCPVTTEC
                     LSWALESGQDAGVWGGMSEDERRALKRRNARTKARTGV"