pAP151 vector (V009488)

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LOCUS       V009488                 8772 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009488
VERSION     V009488
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Park AS, Rubin EJ.
  TITLE     Clp-mediated Regulation of WhiB1 is Required for Cell Division in
            Mycobacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Park AS, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2017) Department of Immunology and Infectious
            Diseases, Harvard School of Public Health, 665 Huntington Ave,
            Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 8772)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2017) Department of Immunology and Infectious Diseases,
            Harvard School of Public Health, 665 Huntington Ave, Boston, MA
            02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(37..83)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     359..375
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    395..415
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             complement(451..1074)
                     /codon_start=1
                     /product="TetR"
                     /label="TetR"
                     /note="Tet repressor protein"
                     /protein_id="AWN08190.1"
                     /translation="MSRLDKSKVINSALALGNEVGIEGVTTRKLAQKLGVAQKTLYWHV
                     KNKRALLDALAVEILARHHDYSLPAAGESWQSFLRNNAMSFRRALLRYRDGAKVHLGTR
                     PDEKQYDTVETQLRFMTENGFSLENALYALSAVGHFTLGCVLEDQEHTAAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     regulatory      complement(1107..1600)
                     /regulatory_class="promoter"
     protein_bind    1600..1624
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1799..1823)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1838..4381
                     /gene="clpC1"
                     /label="ATP-dependent Clp protease ATP-binding subunit
                     ClpC1"
                     /note="ATP-dependent Clp protease ATP-binding subunit ClpC1
                     from Mycobacterium tuberculosis (strain ATCC 25618 /
                     H37Rv). Accession#: P9WPC9"
     polyA_signal    4801..4935
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5131..5719)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6054..6866)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(7150..8262)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"